Food plate samples composed of polystyrene paper (PSP) materials were analyzed for endocrine-disrupting chemical (EDC) using gas chromatography equipped with a flame ionization detector (GC/FID). For the analysis, the frozen filtrating method (FFM) was proposed as a sample pretreatment technique, followed by tert.-butyldimethylsilyl (TBDMS) derivatization. The EDC in food plate samples composed of polystyrene paper material was extracted with acetonitrile and then frozen at −60°C for 2h. The extracts were subsequently filtered and converted to TBDMS derivatives for sensitive analysis by GC/FID. When this method was applied to food plate samples composed of polystyrene paper material, the combination of FFM and TBDMS derivatization proved to be suitable for pharmaceutical-level quality control analysis.