According to recent statistical data released by Statistics Korea, Korea has entered a super-aged society, with the population aged 65 and over steadily increasing. Along with this aging trend, the incidence of metabolic diseases in the elderly is also increasing. Among these diseases, osteoporosis, a disease that causes fractures and joint damage, and menopause, which reduces the quality of life in women, remain significant concerns for many older adults. While various medications and treatments are available, they can cause side effects such as increased risk of cancers like breast cancer and digestive problems. To address these issues, this study aimed to evaluate the efficacy of Carthamus tinctorius L callus-derived exosomes in alleviating osteoporosis and menopausal symptoms in women. In this study, we performed exosome cytotoxicity assessments, TRAP staining, and mRNA expression analysis of genes related to female menopause. The results showed that exosomes did not exhibit significant cytotoxicity in any of the diseases, and TRAP staining confirmed their inhibitory effect on osteoclast differentiation. Finally, RT-qPCR analysis revealed an increased expression of the estrogen receptor (ER-α) gene, a female hormone. In conclusion, this study suggests that Carthamus tinctorius L callus-derived exosomes can improve metabolic disorders associated with osteoporosis and menopause in women.