Obesity is a chronic metabolic disorder characterized by excessive accumulation of body fat and is a major risk factor for various diseases, including type 2 diabetes, hypertension, and cardiovascular diseases. This study investigated the anti-obesity effects of Cannabis sativa extracts (CEs) obtained using various ethanol concentrations. Anti-obesity mechanisms of the extracts were further determined through RT-qPCR and Western blot analysis to evaluated gene and protein expression levels. Differentiation of 3T3-L1 cells was concentration-dependently inhibited by CE at all ethanol concentrations. Especially, the expression of factors involved in lipolysis and white adipose tissue browning, such as HSL, ATGL, UCP1, and PGC-1α, was markedly increased by CE treatment. These effects were enhanced in an ethanol concentration-dependent manner. In conclusion, these results demonstrate that Cannabis sativa effectively mitigates obesity by stimulating lipolysis and white adipose tissue browning.