Staphylococcus aureus​ is a prominent human pathogen that causes an array of infections where the prognosis depends on both bacterial virulence and host immune defense. Therefore, effective treatment requires not only eliminating the pathogen but also modulating the host immune response. Here, we report that the small molecule PM-07 eliminates both growing and persister cells of MRSA by disrupting bacterial membrane, including disrupted proton motive force (PMF) and increased reactive oxygen species (ROS). PM-07 in combination with gentamicin exhibits enhanced anti-persister activity. This agent showed selectivity for bacterial membrane over mammalian cells. Furthermore, PM-07 suppressed the expression of pro-inflammatory mediators (IL-1β, IL-6, and TNF-α) in bacteria-stimulated RAW264.7 cells. Together, these findings highlight the potential of PM-07 as a dual-function therapeutic candidate for MRSA infections by simultaneously eliminating persisters and modulating excessive inflammation.