Inflammation is a fundamental biological response to various pathological stimuli and is largely mediated by pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), as well as enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Lipopolysaccharides (LPS), a component of gram-negative bacteria cell wals, activate toll-like receptor 4 (TLR4), stimulating MAP kinase and NF-κB signaling pathways, thereby increasing the expression of these cytokines and enzymes. These processes contribute to chronic inflammation and metabolic dysfunction, highlighting the importance of targeting these mediators in inflammatory diseases. K-I&T extract, derived from the edible insect Gryllus bimaculatus, has been reported to exert anti-inflammatory effects, suggesting its potential as a functional food or therapeutic agent for managing chronic inflammation. In this study, we evaluated the effects of K-I&T on LPS-induced inflammatory responses in RAW264.7 macrophages. The results demonstrated that K-I&T significantly downregulated the production of TNF-α, IL-6, iNOS, and COX-2, indicating its potential role in mitigating inflammation. Taken together, these findings suggest that K-I&T has potential as a functional food or therapeutic agent for managing chronic inflammatory diseases. This research was supported by the Regional Innovation System & Education(RISE) program through the Jeonbuk RISE Center, funded by the Ministry of Education(MOE) and the Jeonbuk State, Republic of Korea.(2025-RISE-13-WSU)