Multidrug-resistant (MDR) Pseudomonas aeruginosainfection represents a critical challenge in wound management due to impaired healing and limited treatment options. In this study, the therapeutic efficacy of Halcarin 1 was evaluated in a murine wound infection model. Treatment with Halcarin 1 significantly promoted wound closure in a dose-dependent manner, with 150 µM achieving approximately 82% wound area reduction, comparable to polymyxin B (100 µM). Bacterial colonization assays revealed marked suppression of MDR P. aeruginosacounts in wound tissue following Halcarin 1 treatment (100–150 µM). Histological analyses further demonstrated enhanced epithelialization, reduced wound edge, and improved tissue regeneration in the Halcarin 1 group, while Masson’s trichrome staining indicated significantly increased collagen deposition by day 14 compared with PBS controls. Collectively, these findings indicate that Halcarin 1 exerts both potent antibacterial and wound healing activities against MDR P. aeruginosa, highlighting its potential as a promising therapeutic agent for infected wound treatment.