Pancreatic cancer is an aggressive malignancy with high metastatic potential and limited therapeutic options. A key step in metastasis involves survival and growth of cancer cells under anchorage-independent conditions. In this study, we investigated the metabolic features associated with anoikis resistance in pancreatic cancer cells, with a particular focus on glutamine-related pathways. Our comparative analysis revealed distinct alterations in cellular behavior and metabolic dependency that support survival, motility, and invasive properties under detachment conditions. Preliminary findings suggest that glutamine utilization plays a role in sustaining energy homeostasis and cell viability during anchorage-independent growth. These results highlight the potential importance of glutamine-linked metabolic adaptation in metastatic progression and may provide new perspectives for therapeutic intervention.