Goji berries (Lycium chinense Miller and L. barbarum L., Solanaceae) have long been used in traditional medicine, primarily as tea for vision health. However, scientific evidence regarding the bioactive compounds present in their aqueous extracts remains limited. This study aims to determine whether carotenoids, particularly zeaxanthin dipalmitate-one of the major carotenoid-are present in traditionally prepared goji berry tea    and to assess the potential benefits of its consumption. The corroboration of zeaxanthin dipalmitate was achieved through its absorption spectrum and triple quadrupole mass spectrometry. HPLC analysis confirmed the presence of zeaxanthin dipalmitate in both roasted and raw goji berry aqueous extracts, with higher concentrations in L. barbarum. A cytotoxicity assay demonstrated that zeaxanthin dipalmitate provided significant protection against A2E-induced toxicity in ARPE-19 cells, suggesting its potential role in retinal protection. These finding indicate that zeaxanthin dipalmitate can be extracted in aqueous preparations, contrary to the common belief that carotenoids require organic solvents. Additionally, its cytoprotective effects against A2E-induced oxidative stress support the potential therapeutic benefits of goji berry tea for age related macular degeneration (AMD).