Titanium and its alloys are widely used in biomedical implants; however, certain surface characteristics such as low wettability, insufficient surface roughness, and poor pre-implant infections, may lead to implant rejection. Various techniques have been developed to improve the surface biofunction of titanium implants, including physical absorption or the complex chemical modification processes. In this study, we provide the surface modification of titanium implants with biomaterials like collagen layer-by-layer attachable polydopamine(PDA)-coated minocycline poly (lactic-co-glycolic acid) (PLGA) microspheres to enhance the characterization and biofunction of the titanium implants. Minocycline (MINO) is a tetracycline antibiotic that is widely used for treating periodontitis by inhibiting inflammation and repairing the alveolar bone. The result showed that MINO was well encapsulated within PLGA microspheres (3-8 µm) with an approximate loading capacity of 1%. The FT-IR and SEM analyses confirmed the presence of a PDA-coated layer on the microspheres. The layer-by-layer coating process successfully deposited collagen and PDA-coated minocycline PLGA microspheres onto titanium surfaces, which presents a promising approach for enhancing the interfacial osseointegration in titanium implants.