Menopausal women are increased risk of osteoporosis and hot flashes due to decreased estrogen levels. Hormone replacement therapy (HRT) is commonly used to alleviate these symptoms, Due to concerns over the long-term risks of HRT, plant derived phytoestrogens have gained attention as potentially safer alternatives. SB-R is a medicinal plant known for anti-inflammatory and antioxidant properties. Alternative therapies for menopausal symptom relief of SB-R in MCF-7 cells and examined its influence on estrogen receptor alpha (ERα) and related intracellular signaling pathways. The E-Screen assay identified the estrogenic activity-like proliferative effect of SB-R, and Western blot analysis revealed increased expression of ERα along with enhanced phosphorylation of its downstream signaling proteins Akt and Erk. SB-R treatment upregulated the expression of cell cycle regulators including Cyclin E1, Cyclin D1, CDK2, and CDK4. Fluorescence microscopy indicated the translocation of ERα from the cytoplasm to the nucleus following SB-R treatment, investigate its role in transcriptional regulation. These findings indicate that SB-R exhibits estrogen activity through ERα-mediated signaling pathways and cell cycle regulation, supporting its potential as a natural alternative to HRT. This study describes SB-R as a promising candidate for enhancing menopausal health while reducing the risks associated with standard hormone therapy.