The growing demand and expanding markets for essential oils present new opportunities for forestry biomass. The study investigated the physical properties and antimicrobial activity of Lauraceae essential oils against Staphylococcus aureus and Klebsiella pneumoniae. Leaf essential oils (Cinnamomum japonicum Siebold, Neolitsea aciculata (Blume) Koidz., and Neolitsea sericea (Blume) Koidz.) were extracted by hydrodistillation and analysed by gas chromatography/mass spectrometry (GC/MS). Furthermore, the relative density and refractive index of oils were measured according to ISO standards. The relative density and refractive index of Lauraceae essential oils varied 0.876 ± 0.000 - 0.885 ± 0.000 g/ml and 1.4771 ± 0.0002 - 1.5020 ± 0.0001 nD²⁰, respectively. The oil yields varied from 0.28 ± 0.09 - 0.57 ± 0.06 %. C. japonicum oil had nearly twice the yield (0.57 ± 0.06 %) of N. aciculate oil (0.28 ± 0.09 %). The major chemical components of C. japonicum oil were cymene (45.79%), followed by 1-heptanol (16.47%). In particular, 1-heptanol was the predominant component in N. aciculate and N. sericea essential oils (30.50% and 56.28%). The strong antimicrobial activity of N. sericea oil might be due to 1-heptanol (56.28%). N. sericea oil was particularly effective in inhibiting both bacterial growths (MIC: 1-1.5% and MBC: 3%), by suggesting the promising antibiotics.