Salvia miltiorrhiza (S. miltiorrhiza) Bunge has traditionally been used for cardiovascular and cerebrovascular diseases. The active compounds cryptotanshinone (CT) and tanshinone IIA (TSA) isolated from S. miltiorrhiza root are have various effects such as anti-inflammatoty, anti-cancer, neuroprotective and cardiprotective. This study was designed to conduct the quantitative analysis of CT and TSA from S. miltiorrhiza root according to extraction conditions. The powdered S. miltiorrhiza was extracted according to the different extraction method, solvent, and temperature. High-performance liquid chromatography (HPLC) was used for validation and quantitative analysis of CT and TSA in S. miltiorrhiza extract (SME). The HPLC analysis with SME was performed by the validated method. The contents of both CT and TSA were the highest when the extraction was performed with 100% ethanol in pecolation method. Additionally, as the temperature was increased during reflux extraction, the contents of CT and TSA were decreased. The results of this study using HPLC can be used as basic data for quantitative analysis of S. miltiorrhiza product for the prevention and treatment of cardiovascular and cerebrovascular diseases.