Discovery and biosynthesis of specialized metabolites from the human blood-derived bacteria, Paracoccus sanguinis
While various metabolic studies are underway to explore the human microbiome, research in this area remains notably scarce compared to the abundance of microbial diversity. Specifically, within the realm of human microbiota, blood microbial communities have been particularly overlooked, with no studies conducted at the level of individual metabolites to date. Here we investigated with Paracoccus sanguinis, isolated from human blood, which was suggested to have biosynthetic genes for thioamide-NRP and ranthipeptide family by antiSMASH analysis. In this study, we isolated six novel secondary metabolites including five indole derivatives, and their chemical structures were elucidated by NMR and HR-TOF-MS. Also, we proposed a biosynthetic pathway through biomimetic total synthesis and labeled 13C amino acids feeding study.
2024 Spring Convention