New lipopeptides from the marine bacteria Pseudovibrio denitrificans
Microorganisms inhabiting distinctive and underexplored environments represent a rich source of bioactive compounds characterized by unique structures. Similarly, marine bacteria have garnered considerable interest due to their potential to yield unprecedented bioactive compounds. In this study, we isolated eight lipopeptides (1–8) from the seawater-derived bacterium Pseudovibrio denitrificans KCTC 62704. The chemical structures of the new compounds (3–8) were characterized by spectroscopic and spectrometric data interpretation, including NMR (1H and 13C NMR, 1H–1H COSY, HSQC, HMBC, and NOESY) and HRESIMS analysis. Experimental ECD data analysis was conducted to assign the absolute configurations of the new compounds. We propose that these lipopeptides are synthesized from hexanoic acid, L-phenylalanine, and additional building blocks by stable-isotope feeding experiments. AntiSMASH analysis of the genome of P. denitrificans KCTC 62704 revealed the potential biosynthetic gene cluster for the isolated lipopeptides.
2024 Spring Convention