Hijikia fusiforme (HF), a member of brown algae family, exerts various pharmacological effects, including preventing arteriosclerosis and menopausal disorders. This study aimed to elucidate the ameliorative effect of HF on atopic dermatitis (AD) under in vivo conditions. We examined the pharmacological effects of HF on compound 48/80- or histamine-induced scratching behaviors and 2, 4-dinitrochlrobenzene (DNCB)-induced AD-like skin lesions in mice. The finding of this study demonstrated that HF attenuated the AD symptoms such as itching, eczematous, erythema and dryness and serum IgE and histamine levels in mice. In addition, HF inhibited the levels of inflammatory cytokines and NF-κB activation in AD-like skin lesion in mice. Taken together, these results demonstrate that HF may be a useful therapeutic agent for treatment of AD and skin inflammation.